The effects of diethylstilbestrol administration on rat kidney. Ultrastructural study.pdf

نأ نكيم يتلا ةيبيكرتلاو ةيجيسنلا تاريغتلا مييقت :فادهلأا نم ىلكلا ةجسنأ ىلع لورتسيبليتسليثيا ياد راقع اهببسي .ةيبيكرتو ةيعانمو ةيجيسن ةسارد للاخ ،بطلا ةيلك ،حيرشتلا مسق يف ةساردلا هذه تيرجأ :ةقيرطلا تتم ةيدوعسلا ةيبرعلا ةكلملما ،ةدج ،زيزعلادبع كللما ةعماج 30 يلاوح .م2012 ربمسيد ىلإ م2011 ربمسيد نم ةرتفلا يف لكل ناذرج 10( تاعومجم 3 ىلإ مهميسقت تم اًغلاب ًاركذ ًاذرج ةيناثلا ةعومجلماو ،ةطباضلا يه ىلولأا ةعومجلما ،)ةعومجم لكل مارجوركيم 60 ةعرجب لورتسيبليتسليثيا ياد راقع تقلت 20 ةدلم ةرذلا تيز نم لم 0.1 يف مويلا يف ذرلجا نزو نم مجك لورتسيبليتسليثيا ياد راقع تقلت ةثلاثلا ةعومجلما امنيب ،اًموي 0.1 يف مويلا يف ذرلجا نزو نم مجك لكل مارجوركيم 60 ةعرجب ةجسنأ ةسارد تم .مفلا قيرط نع اًموي 50 ةدلم ةرذلا تيز نم لم ةساردلا تيرجأ دقو .ينورتكللإا رهجلماو يئوضلا رهجلماب ىلكلا .زيزعلا دبع كللما ةعماج ،بطلا ةيلك ،حيرشتلا مسق يف اًموي 50 ةدلم لورتسيبليتسليثيا ياد راقع ىطاعت نأ :جئاتنلا ىلكلل ةيبيكرتلا ةجسنلأا يف ةظوحلم تارييغت يف ببست كانه تناك ،كلذ ىلع ةولاعو .ةيولكلا بيبانلأا فلت رهظو لقأ تاريغتلا هذه لك تناكو .ةيعانلما ماسجلأا يف ةريبك ةدايز .جلاعلا نم اًموي 20 دعب اًحوضو ىلع ةراض راثآ ةيعانطصلاا ينجورتسلاا تابكرم ترهظأ :ةتماخ ىلع دمتعت تارييغتلا هذهو اهبيكرت يف رييغتو ىلكلا ةجسنأ .يطاعتلا ةدم Objectives: To assess the histological and ultrastructural changes that can be induced by diethylstilbestrol (DES) on renal tissues using histological, immunohistochemical, and ultrastructural methods. Methods: Thirty adult male Wistar rats were divided into 3 groups (10 rats each): Group 1 control; Group 2 received DES at a dose of 60 μg/kg/day, dissolved in 0.1 ml corn oil for 20 days; and Group Articles 3 received the same dose of DES for 50 days by oral gavage. The renal tissues were studied histologically, immunohistochemically (using an anti-BCL2associated X protein [BAX protein] antibody), and ultrastructurally. This study was carried out at the Anatomy Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia between December 2011 and December 2012. Results: The DES administration for 50 days caused noticeable degeneration, and alteration of the morphology of the renal tissues in the form of damaged renal tubules with loss of the brush border of the proximal convoluted tubules and increased cellularity of the glomeruli. In addition, there was a significant increase in BAX protein expression based on immunoreactivity, and in renal tubules, as well as glomerular cells. These changes were less obvious after 20 days of treatment. Conclusion: Non-steroidal, synthetic estrogens showed harmful effects on the renal tissues and altered their morphology with an increased number of apoptotic cells, and these changes were duration dependent. Saudi Med J 2013; Vol. 34 (11): 1114-1124 From the Anatomy Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia. Received 29th April 2013. Accepted 2nd September 2013. Address correspondence and reprint request to: Dr. Mustafa H. Noaman, Anatomy Department, Faculty of Medicine, Ground Floor, Building No. 8, King Abdulaziz University, PO Box 80205, Jeddah 21589, Kingdom of Saudi Arabia. Tel. +966 566764762. E-mail: [email protected] 1114 Saudi Med J 2013; Vol. 34 (11) www.smj.org.sa Disclosure. This study was funded by the Deanship of Scientific Research (DSR) (grant #6-140-D 1432), King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.